본문영역
|
세미나 안내 - 연사 : Dr. Jung Sun Yoo (2012. 4. 9. 월요일 16:00)
작성자 : 관리자작성일 :2012-03-27 18:04:57조회수 : 3829
: 전체
|
|||||
|---|---|---|---|---|---|
|
Title : Monocyte/Macrophage Specific Fluorescent Probe Development to Assess Inflammation In Vivo Speaker : Dr. Jung Sun Yoo (Research Fellow, Laboratory of Bioimaging Probe Development, Department of Chemistry, National University of Singapore & Singapore Bioimaging Consortium, A*STAR) Date & Time : 2012. 4. 9. 월요일 16:00 Abstract Inflammation plays a central role in the pathophysiology of numerous diseases. Inflammation directly participates from the very inception to the progression of not only representative inflammatory diseases such as rheumatoid arthritis and osteoarthritis but also more lethal diseases such as atherosclerosis, cancer and Alzheimer’s disease. In recent years, visualization of inflammation has received particular attention to understand qualitative aspects of these diseases. For example, it was believed that atherosclerosis was merely passive accumulation of cholesterol in the vessel wall for many years. Today, the picture is much more complex, with atherosclerosis being thought of as a chronic inflammatory disease. Monocyte/Macrophage has been a major target for in vivo inflammation imaging since it reveals centers of inflammation in a patient and, thereby, contributes to diagnosis and therapeutic assessment. The 99mTc-HMPAO method has been widely used over the last 25years as a “gold standard” for the imaging of inflammation. Because the labelled cells with 99mTc are re-injected into the patient, there is a lot of risk such as contamination of the blood and exposing the operator to blood-borne pathogens.Therefore, the development of a new tracer to track macrophages infiltrating inflamed area using direct administration will provide significant impact the ways of in vivo imaging and quantification of inflammation in various diseases. Our group has developed by combinatorial chemistry the libraries of several thousands of small molecules that resemble natural products in their complexity and possess significant skeletal and stereochemical diversity. Using this collection, we screened the fluorescent libraries comprising 1706 compounds against different blood cell lines and discovered a series of probes to stain monocyte/macrophage specifically at different wavelength regions. The hit (BDNCA2-A8) from the BODIPY scaffold based library screen was tested with fresh human peripheral leukocytes and human adipose tissue derived cells to show high specificity for monocyte/macrophage depicting robustness as a targeted live cell imaging and isolation fluorophore. The near-infrared fluorescent CyRCA-A4 probe was shown to target monocyte/macrophage in inflamed lesion in vivo utilizing the favourable optical properties of the NIR window, namely, relatively limited photon absorption by blood and reduced tissue autofluorescence. The binding of the NIR probe to the sites of inflammation was determined to be specific, and we validated this specific binding by successful in vivo visualization of LPS injected mouse models as well as hind-limb ischemia models with a high signal-to-background ratio. The developed monocyte/macrophage specific probes using diversity-oriented synthesis approach showcased the potential for highly selective biological imaging and improved clinical staging of inflammation. ※ 자세한 내용은 첨부문서를 참고해 주시기 바랍니다. |
|||||
| 영상주소 | |||||
| 첨부파일 | 12.04.09_융기원_dr._jung_sun_yoo.doc [69건 다운로드] |
||||
| 이전글 | 융대원 나노융합학과 세미나 안내 - 연사 : Prof. Siegmar Roth 2012-03-26 |
|---|---|
| 현재글 | 세미나 안내 - 연사 : Dr. Jung Sun Yoo (2012. 4. 9. 월요일 16:00) 2012-03-27 |
| 다음글 | (채용공고)차세대융합기술연구원 직원 채용 공고 2012-03-28 |
